Effects of Specific Amino Acid Substitutions on Activities of Dinitrogenase Reductase-Activating Glycohydrolase from Rhodospirillum rubrum

Site-directed mutagenesis of the draG gene was used to generate altered forms of dinitrogenase reductase-activating glycohydrolase (DRAG) with D123A, H142L, H158N, D243G, and E279R substitutions. The amino acid residues H142 and E279 are not required either for the coordination to the metal center o...

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Bibliografiske detaljer
Main Authors: Antharavally, Babu S., Poyner, Russell R., Zhang, Yaoping, Roberts, Gary P., Ludden, Paul W.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
Fag:
Enzymes and Proteins
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC95467/
https://ncbi.nlm.nih.gov/pubmed/11544238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.19.5743-5746.2001
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