Monitoring Intracellular Levels of XylR in Pseudomonas putida with a Single-Chain Antibody Specific for Aromatic-Responsive Enhancer-Binding Proteins

We have isolated a recombinant phage antibody (Phab) that binds a distinct epitope of the subclass of the ς(54)-dependent prokaryotic enhancer-binding proteins that respond directly to aromatic effectors, e.g., those that activate biodegradative operons of Pseudomonas spp. The DNA segments encoding...

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Detalhes bibliográficos
Main Authors: Fraile, Sofía, Roncal, Fernando, Fernández, Luis A., de Lorenzo, Víctor
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC95448/
https://ncbi.nlm.nih.gov/pubmed/11544219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.19.5571-5579.2001
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