Directed Evolution of Biphenyl Dioxygenase: Emergence of Enhanced Degradation Capacity for Benzene, Toluene, and Alkylbenzenes

Biphenyl dioxygenase (Bph Dox) catalyzes the initial oxygenation of biphenyl and related compounds. Bph Dox is a multicomponent enzyme in which a large subunit (encoded by the bphA1 gene) is significantly responsible for substrate specificity. By using the process of DNA shuffling of bphA1 of Pseudo...

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Bibliografiske detaljer
Main Authors: Suenaga, Hikaru, Mitsuoka, Mariko, Ura, Yuko, Watanabe, Takahito, Furukawa, Kensuke
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
Fag:
Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC95430/
https://ncbi.nlm.nih.gov/pubmed/11514531
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.18.5441-5444.2001
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