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New Plasmid System To Select for Saccharomyces cerevisiae Purine-Cytosine Permease Affinity Mutants
The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mu...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC95330/ https://ncbi.nlm.nih.gov/pubmed/11418581 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.14.4386-4388.2001 |
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