Mutations in Genes Involved in the Flagellar Export Apparatus of the Solvent-Tolerant Pseudomonas putida DOT-T1E Strain Impair Motility and Lead to Hypersensitivity to Toluene Shocks

Pseudomonas putida DOT-T1E is a solvent-tolerant strain able to grow in the presence of 1% (vol/vol) toluene in the culture medium. Random mutagenesis with mini-Tn5-′phoA-Km allowed us to isolate a mutant strain (DOT-T1E-42) that formed blue colonies on Luria-Bertani medium supplemented with 5-bromo...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Segura, Ana, Duque, Estrella, Hurtado, Ana, Ramos, Juan L.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Cell Surfaces
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC95300/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11418551/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.14.4127-4133.2001
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