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Purification of the RelB and RelE Proteins of Escherichia coli: RelE Binds to RelB and to Ribosomes

The direct interaction of the Escherichia coli cytotoxin RelE with its specific antidote, RelB, was demonstrated in two ways: (i) copurification of the two proteins and (ii) a positive yeast two-hybrid assay involving the relB and relE genes. In addition, the purified RelE protein exhibited ribosome...

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Autori principali: Galvani, Cheryl, Terry, Jefferson, Ishiguro, Edward E.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2001
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC95192/
https://ncbi.nlm.nih.gov/pubmed/11274135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.8.2700-2703.2001
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