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Topology of OxlT, the Oxalate Transporter of Oxalobacter formigenes, Determined by Site-Directed Fluorescence Labeling

The topology of OxlT, the oxalate:formate exchange protein of Oxalobacter formigenes, was established by site-directed fluorescence labeling, a simple strategy that generates topological information in the context of the intact protein. Accessibility of cysteine to the fluorescent thiol-directed pro...

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Detalhes bibliográficos
Main Authors: Ye, Liwen, Jia, Zhenzhen, Jung, Thomas, Maloney, Peter C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC95165/
https://ncbi.nlm.nih.gov/pubmed/11274108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.8.2490-2496.2001
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