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Altering Catalytic Properties of 3-Chlorocatechol-Oxidizing Extradiol Dioxygenase from Sphingomonas xenophaga BN6 by Random Mutagenesis

The 2,3-dihydroxybiphenyl 1,2-dioxygenase from Sphingomonas xenophaga strain BN6 (BphC1) oxidizes 3-chlorocatechol by a rather unique distal ring cleavage mechanism. In an effort to improve the efficiency of this reaction, bphC1 was randomly mutated by error-prone PCR. Mutants which showed increased...

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Sonraí Bibleagrafaíochta
Main Authors: Riegert, Ulrich, Bürger, Sibylle, Stolz, Andreas
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 2001
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC95140/
https://ncbi.nlm.nih.gov/pubmed/11244073
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.7.2322-2330.2001
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