Altering Catalytic Properties of 3-Chlorocatechol-Oxidizing Extradiol Dioxygenase from Sphingomonas xenophaga BN6 by Random Mutagenesis

The 2,3-dihydroxybiphenyl 1,2-dioxygenase from Sphingomonas xenophaga strain BN6 (BphC1) oxidizes 3-chlorocatechol by a rather unique distal ring cleavage mechanism. In an effort to improve the efficiency of this reaction, bphC1 was randomly mutated by error-prone PCR. Mutants which showed increased...

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Manylion Llyfryddiaeth

Cyhoeddwyd yn:	J Bacteriol
Prif Awduron:	Riegert, Ulrich, Bürger, Sibylle, Stolz, Andreas
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	American Society for Microbiology (ASM) 2001
Pynciau:	Enzymes and Proteins
Mynediad Ar-lein:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC95140/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11244073/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.7.2322-2330.2001
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