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The Initiation Codon Affects Ribosome Binding and Translational Efficiency in Escherichia coli of cI mRNA with or without the 5′ Untranslated Leader

Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage λ. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was ba...

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Udgivet i:J Bacteriol
Principais autores: O'Donnell, Sean M., Janssen, Gary R.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2001
Fag:
Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC95001/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11157940/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.4.1277-1283.2001
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