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Effect of Sequences of the Active-Site Dipeptides of DsbA and DsbC on In Vivo Folding of Multidisulfide Proteins in Escherichia coli

We have examined the role of the active-site CXXC central dipeptides of DsbA and DsbC in disulfide bond formation and isomerization in the Escherichia coli periplasm. DsbA active-site mutants with a wide range of redox potentials were expressed either from the trc promoter on a multicopy plasmid or...

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Detalhes bibliográficos
Main Authors: Bessette, Paul H., Qiu, Ji, Bardwell, James C. A., Swartz, James R., Georgiou, George
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC94966/
https://ncbi.nlm.nih.gov/pubmed/11208797
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.3.980-988.2001
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