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Sensitive Genetic Screen for Protease Activity Based on a Cyclic AMP Signaling Cascade in Escherichia coli

We describe a genetic system that allows in vivo screening or selection of site-specific proteases and of their cognate-specific inhibitors in Escherichia coli. This genetic test is based on the specific proteolysis of a signaling enzyme, the adenylate cyclase (AC) of Bordetella pertussis. As a mode...

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Main Authors: Dautin, Nathalie, Karimova, Gouzel, Ullmann, Agnes, Ladant, Daniel
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 2000
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC94834/
https://ncbi.nlm.nih.gov/pubmed/11092869
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