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Sensitive Genetic Screen for Protease Activity Based on a Cyclic AMP Signaling Cascade in Escherichia coli
We describe a genetic system that allows in vivo screening or selection of site-specific proteases and of their cognate-specific inhibitors in Escherichia coli. This genetic test is based on the specific proteolysis of a signaling enzyme, the adenylate cyclase (AC) of Bordetella pertussis. As a mode...
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| Main Authors: | , , , |
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| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
American Society for Microbiology
2000
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC94834/ https://ncbi.nlm.nih.gov/pubmed/11092869 |
| Clibeanna: |
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