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A Single-Transformation Gene Function Test in Diploid Candida albicans

The fungal pathogen Candida albicans is naturally diploid, and current gene disruption strategies require two successive transformations. We describe here a genetic construct (UAU1) for which two copies may be selected. Insertion of UAU1 into one genomic site, after a single transformation, allows s...

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Main Authors: Enloe, Brian, Diamond, Aviva, Mitchell, Aaron P.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2000
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC94694/
https://ncbi.nlm.nih.gov/pubmed/11004171
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