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Visualization of Repair of Double-Strand Breaks in the Bacteriophage T7 Genome without Normal DNA Replication

An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 is able to repair double-strand breaks in a T7 genome with efficiencies of 20% or more. To achieve this high repair efficiency it is necessary that the reaction mixtures contain molecules of donor DNA that bracke...

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Detalles Bibliográficos
Main Authors: Lai, Ying-Ta, Masker, Warren
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2000
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC94280/
https://ncbi.nlm.nih.gov/pubmed/10629177
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