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Gene Disruption through Homologous Recombination in Spiroplasma citri: an scm1-Disrupted Motility Mutant Is Pathogenic

To determine whether homologous recombination could be used to inactivate selected genes in Spiroplasma citri, plasmid constructs were designed to disrupt the motility gene scm1. An internal scm1 gene fragment was inserted into plasmid pKT1, which replicates in Escherichia coli but not in S. citri,...

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Bibliographische Detailangaben
Hauptverfasser: Duret, Sybille, Danet, Jean-Luc, Garnier, Monique, Renaudin, Joël
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1999
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC94200/
https://ncbi.nlm.nih.gov/pubmed/10601200
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