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Proton-Nuclear Magnetic Resonance Analyses of the Substrate Specificity of a β-Ketolase from Pseudomonas putida, Acetopyruvate Hydrolase

A revised purification of acetopyruvate hydrolase from orcinol-grown Pseudomonas putida ORC is described. This carbon-carbon bond hydrolase, which is the last inducible enzyme of the orcinol catabolic pathway, is monomeric with a molecular size of ∼38 kDa; it hydrolyzes acetopyruvate to equimolar qu...

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Detalhes bibliográficos
Main Authors: Pokorny, Diana, Brecker, Lothar, Pogorevc, Mateja, Steiner, Walter, Griengl, Herfried, Kappe, Thomas, Ribbons, Douglas W.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 1999
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC93995/
https://ncbi.nlm.nih.gov/pubmed/10438778
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