Site-Directed Mutagenesis of Loop L3 of Sucrose Porin ScrY Leads to Changes in Substrate Selectivity

The difference in substrate selectivity of the maltodextrin (LamB) and sucrose (ScrY) porins is attributed mainly to differences in loop L3, which is supposed to constrict the lumen of the pores. We show that even a single mutation (D201Y) in loop L3 leads to a narrowing of the substrate range of Sc...

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הוצא לאור ב:	J Bacteriol
Principais autores:	Ulmke, Christine, Kreth, Jens, Lengeler, Joseph W., Welte, Wolfram, Schmid, Kurt
פורמט:	Artigo
שפה:	Inglês
יצא לאור:	American Society for Microbiology (ASM) 1999
נושאים:	Physiology and Metabolism
גישה מקוונת:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93594/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10074088/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.6.1920-1923.1999
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