Immunochemical Analysis of UMP Kinase from Escherichia coli

Mono- and polyclonal antibodies directed against UMP kinase from Escherichia coli were tested with the intact protein or with fragments obtained by deletion mutagenesis. As detected in enzyme-linked immunosorbent assay tests, the carboxy-terminal quarter of UMP kinase is immunodominant. Polyclonal a...

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Bibliographic Details

Published in:	J Bacteriol
Main Authors:	Landais, Stéphanie, Gounon, Pierre, Laurent-Winter, Christine, Mazié, Jean-Claude, Danchin, Antoine, Bârzu, Octavian, Sakamoto, Hiroshi
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 1999
Subjects:	Enzymes and Proteins
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93449/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9922246/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.3.833-840.1999
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