Purification and Properties of NADH-Dependent 5,10-Methylenetetrahydrofolate Reductase (MetF) from Escherichia coli

A K-12 strain of Escherichia coli that overproduces methylenetetrahydrofolate reductase (MetF) has been constructed, and the enzyme has been purified to apparent homogeneity. A plasmid specifying MetF with six histidine residues added to the C terminus has been used to purify histidine-tagged MetF t...

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Bibliografische Detailangaben

Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Sheppard, Christal A., Trimmer, Elizabeth E., Matthews, Rowena G.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1999
Schlagworte:	Enzymes and Proteins
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93435/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9922232/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.3.718-725.1999
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