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Detection and Enumeration of Methanotrophs in Acidic Sphagnum Peat by 16S rRNA Fluorescence In Situ Hybridization, Including the Use of Newly Developed Oligonucleotide Probes for Methylocella palustris

Two 16S rRNA-targeted oligonucleotide probes, Mcell-1026 and Mcell-181, were developed for specific detection of the acidophilic methanotroph Methylocella palustris using fluorescence in situ hybridization (FISH). The fluorescence signal of probe Mcell-181 was enhanced by its combined application wi...

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Bibliografiske detaljer
Main Authors: Dedysh, Svetlana N., Derakshani, Manigee, Liesack, Werner
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC93240/
https://ncbi.nlm.nih.gov/pubmed/11571193
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.10.4850-4857.2001
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