Effect of Primers Hybridizing to Different Evolutionarily Conserved Regions of the Small-Subunit rRNA Gene in PCR-Based Microbial Community Analyses and Genetic Profiling

Genetic profiling techniques of microbial communities based on PCR-amplified signature genes, such as denaturing gradient gel electrophoresis or single-strand-conformation polymorphism (SSCP) analysis, are normally done with PCR products of less than 500-bp. The most common target for diversity anal...

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Detalhes bibliográficos
Principais autores: Schmalenberger, Achim, Schwieger, Frank, Tebbe, Christoph C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC93056/
https://ncbi.nlm.nih.gov/pubmed/11472932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.8.3557-3563.2001
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