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Development of a Quantitative Competitive PCR Assay for Detection and Quantification of Escherichia coli O157:H7 Cells

A quantitative competitive PCR (QC-PCR) assay was developed to detect and quantify Escherichia coli O157:H7 cells. From 10(3) to 10(8) CFU of E. coli O157:H7 cells/ml was quantified in broth or skim milk, and cell densities predicted by QC-PCR were highly related to viable cell counts (r(2) = 0.99 a...

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Publicado en:Appl Environ Microbiol
Autores principales: Li, Wenli, Drake, Mary Anne
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 2001
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93014/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11425755/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.7.3291-3294.2001
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