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Purification of Synechocystis sp. Strain PCC6308 Cyanophycin Synthetase and Its Characterization with Respect to Substrate and Primer Specificity

Synechocystis sp. strain PCC6308 cyanophycin synthetase was purified 72-fold in three steps by anion exchange chromatography on Q Sepharose, affinity chromatography on the triazine dye matrix Procion Blue HE-RD Sepharose, and gel filtration on Superdex 200 HR from recombinant cells of Escherichia co...

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Autors principals: Aboulmagd, Elsayed, Oppermann-Sanio, Fred B., Steinbüchel, Alexander
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC92852/
https://ncbi.nlm.nih.gov/pubmed/11319097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.5.2176-2182.2001
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