Secretion of Recombinant Proteins via the Chaperone/Usher Pathway in Escherichia coli

F1 antigen (Caf1) of Yersinia pestis is assembled via the Caf1M chaperone/Caf1A usher pathway. We investigated the ability of this assembly system to facilitate secretion of full-length heterologous proteins fused to the Caf1 subunit in Escherichia coli. Despite correct processing of a chimeric prot...

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Détails bibliographiques
Auteurs principaux: Zavialov, Anton V., Batchikova, Natalia V., Korpela, Timo, Petrovskaya, Lada E., Korobko, Vyacheslav G., Kersley, Joanne, MacIntyre, Sheila, Zav'yalov, Vladimir P.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2001
Sujets:
Genetics and Molecular Biology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC92801/
https://ncbi.nlm.nih.gov/pubmed/11282637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.4.1805-1814.2001
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