Purification, Characterization, and Gene Cloning of Purine Nucleosidase from Ochrobactrum anthropi

A bacterium, Ochrobactrum anthropi, produced a large amount of a nucleosidase when cultivated with purine nucleosides. The nucleosidase was purified to homogeneity. The enzyme has a molecular weight of about 170,000 and consists of four identical subunits. It specifically catalyzes the irreversible...

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Bibliographic Details
Main Authors: Ogawa, Jun, Takeda, Sou, Xie, Sheng-Xue, Hatanaka, Haruyo, Ashikari, Toshihiko, Amachi, Teruo, Shimizu, Sakayu
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC92797/
https://ncbi.nlm.nih.gov/pubmed/11282633
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.4.1783-1787.2001
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