Expression in Escherichia coli of Native and Chimeric Phenolic Acid Decarboxylases with Modified Enzymatic Activities and Method for Screening Recombinant E. coli Strains Expressing These Enzymes

Four bacterial phenolic acid decarboxylases (PAD) from Lactobacillus plantarum, Pediococcus pentosaceus, Bacillus subtilis, and Bacillus pumilus were expressed in Escherichia coli, and their activities on p-coumaric, ferulic, and caffeic acids were compared. Although these four enzymes displayed 61%...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Barthelmebs, Lise, Diviès, Charles, Cavin, Jean-François
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92695/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11229892/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.3.1063-1069.2001
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