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Nickel-Resistance-Based Minitransposons: New Tools for Genetic Manipulation of Environmental Bacteria

The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre minitransposons in members of the α, β, and γ subclasses of the Proteobacteria, while the ncc minitr...

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Detalhes bibliográficos
Main Authors: Taghavi, Safieh, Delanghe, Hilde, Lodewyckx, Cindy, Mergeay, Max, van der Lelie, Daniël
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92686/
https://ncbi.nlm.nih.gov/pubmed/11157282
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.2.1015-1019.2001
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