Evaluation of PCR-Generated Chimeras, Mutations, and Heteroduplexes with 16S rRNA Gene-Based Cloning

To evaluate PCR-generated artifacts (i.e., chimeras, mutations, and heteroduplexes) with the 16S ribosomal DNA (rDNA)-based cloning approach, a model community of four species was constructed from alpha, beta, and gamma subdivisions of the division Proteobacteria as well as gram-positive bacterium,...

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Bibliographische Detailangaben
Hauptverfasser: Qiu, Xiaoyun, Wu, Liyou, Huang, Heshu, McDonel, Patrick E., Palumbo, Anthony V., Tiedje, James M., Zhou, Jizhong
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2001
Schlagworte:
Methods
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC92662/
https://ncbi.nlm.nih.gov/pubmed/11157258
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.2.880-887.2001
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