Development of a Gene Cloning and Inactivation System for Halorespiring Desulfitobacterium dehalogenans

Efficient host-vector systems have been developed for the versatile, strictly anaerobic, halo- and fumarate-respiring gram-positive bacterium Desulfitobacterium dehalogenans. An electroporation-based transformation procedure resulting in approximately 10(3) to 10(4) transformants per μg of the cloni...

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Main Authors: Smidt, Hauke, van der Oost, John, de Vos, Willem M.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2001
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Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC92625/
https://ncbi.nlm.nih.gov/pubmed/11157221
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.2.591-597.2001
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