Bifidobacterial Diversity in Human Feces Detected by Genus-Specific PCR and Denaturing Gradient Gel Electrophoresis

We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S riboso...

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Bibliografiska uppgifter
Huvudupphovsmän: Satokari, Reetta M., Vaughan, Elaine E., Akkermans, Antoon D. L., Saarela, Maria, de Vos, Willem M.
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 2001
Ämnen:
Methods
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC92614/
https://ncbi.nlm.nih.gov/pubmed/11157210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.2.504-513.2001
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