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General method of rapid Smith/Birnstiel mapping adds for gap closure in shotgun microbial genome sequencing projects: application to Pseudomonas putida KT2440

A physical mapping strategy has been developed to verify and accelerate the assembly and gap closure phase of a microbial genome shotgun-sequencing project. The protocol was worked out during the ongoing Pseudomonas putida KT2440 genome project. A macro-restriction map was constructed by linking pro...

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Bibliographic Details
Main Authors: Weinel, Christian, Tümmler, Burkhard, Hilbert, Helmut, Nelson, Karen E., Kiewitz, Claudia
Format: Artigo
Language:Inglês
Published: Oxford University Press 2001
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC92575/
https://ncbi.nlm.nih.gov/pubmed/11713330
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