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Leaky Lactococcus Cultures That Externalize Enzymes and Antigens Independently of Culture Lysis and Secretion and Export Pathways

A novel system that leaks β-galactosidase (β-gal) without a requirement for secretion or export signals was developed in Lactococcus lactis by controlled expression of integrated phage holin and lysin cassettes. The late promoter of the lytic lactococcal bacteriophage φ31 is an 888-bp fragment (P(15...

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Detalhes bibliográficos
Main Authors: Walker, Shirley A., Klaenhammer, Todd R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92559/
https://ncbi.nlm.nih.gov/pubmed/11133453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.1.251-259.2001
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