Detection of the 5′-cap structure of messenger RNAs with the use of the cap-jumping approach

An effective procedure for specific determination of the cap structure at the 5′-terminus of mRNA and for isolation of the corresponding full-length cDNA has been developed. The procedure involves covalent attachment of an oligonucleotide template extender to the 5′-cap structure of mRNA followed by...

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Main Authors: Efimov, V. A., Chakhmakhcheva, O. G., Archdeacon, J., Fernandez, J. M., Fedorkin, O. N., Dorokhov, Yu. L., Atabekov, J. G.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2001
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC92527/
https://ncbi.nlm.nih.gov/pubmed/11713326
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