Thermostable NADP(+)-Dependent Medium-Chain Alcohol Dehydrogenase from Acinetobacter sp. Strain M-1: Purification and Characterization and Gene Expression in Escherichia coli

NADPH-dependent alkylaldehyde reducing enzyme, which was greatly induced by n-hexadecane, from Acinetobacter sp. strain M-1 was purified and characterized. The purified enzyme had molecular masses of 40 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 160 kDa as det...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Tani, Akio, Sakai, Yasuyoshi, Ishige, Takeru, Kato, Nobuo
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2000
Sujets:	Enzymology and Protein Engineering
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92449/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11097895/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.12.5231-5235.2000
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