Cloning and Random Mutagenesis of the Erwinia herbicola tyrR Gene for High-Level Expression of Tyrosine Phenol-Lyase

Tyrosine phenol-lyase (Tpl), which can synthesize 3,4-dihydroxyphenylalanine from pyruvate, ammonia, and catechol, is a tyrosine-inducible enzyme. Previous studies demonstrated that the tpl promoter of Erwinia herbicola is activated by the TyrR protein of Escherichia coli. In an attempt to create a...

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Bibliographic Details
Main Authors: Katayama, Takane, Suzuki, Hideyuki, Koyanagi, Takashi, Kumagai, Hidehiko
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC92377/
https://ncbi.nlm.nih.gov/pubmed/11055921
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