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The Presence of Humic Substances and DNA in RNA Extracts Affects Hybridization Results

RNA extracts obtained from environmental samples are frequently contaminated with coextracted humic substances and DNA. It was demonstrated that the response in rRNA-targeted oligonucleotide probe hybridizations decreased as the concentrations of humic substances and DNA in RNA extracts increased. T...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Alm, Elizabeth Wheeler, Zheng, Dandan, Raskin, Lutgarde
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2000
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC92341/
https://ncbi.nlm.nih.gov/pubmed/11010915
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