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Antisense Downregulation of ς(32) as a Transient Metabolic Controller in Escherichia coli: Effects on Yield of Active Organophosphorus Hydrolase

Plasmids containing an antisense fragment of the ς(32) gene were constructed and introduced into Escherichia coli cells. Downregulation of the ς(32)-mediated stress response was evaluated under heat shock and ethanol stress and during the production of organophosphorus hydrolase (OPH). Northern blot...

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Detalhes bibliográficos
Main Authors: Srivastava, Ranjan, Cha, Hyung Joon, Peterson, Marvin S., Bentley, William E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92310/
https://ncbi.nlm.nih.gov/pubmed/11010884
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