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Affinity Purification of DNA and RNA from Environmental Samples with Peptide Nucleic Acid Clamps

Bispeptide nucleic acids (bis-PNAs; PNA clamps), PNA oligomers, and DNA oligonucleotides were evaluated as affinity purification reagents for subfemtomolar 16S ribosomal DNA (rDNA) and rRNA targets in soil, sediment, and industrial air filter nucleic acid extracts. Under low-salt hybridization condi...

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Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Chandler, Darrell P., Stults, Jennie R., Cebula, Sharon, Schuck, Beatrice L., Weaver, Derek W., Anderson, Kevin K., Egholm, Michael, Brockman, Fred J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 2000
Schlagworte:
Methods
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92168/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10919804/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.8.3438-3445.2000
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