Transformation of Acinetobacter sp. Strain BD413(pFG4ΔnptII) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of Transformants

Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp-deleted nptII gene in Acinetobacter sp. strain BD413(pFG4) cells incubated in sterile soil microcosms was detected after add...

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Bibliografiske detaljer
Main Authors: Nielsen, Kaare M., van Elsas, Jan D., Smalla, Kornelia
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2000
Fag:
Microbial Ecology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC91972/
https://ncbi.nlm.nih.gov/pubmed/10698801
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