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Selective and Sensitive Method for PCR Amplification of Escherichia coli 16S rRNA Genes in Soil
A set of PCR primers targeting 16S rRNA gene sequences was designed, and PCR parameters were optimized to develop a robust and reliable protocol for selective amplification of Escherichia coli 16S rRNA genes. The method was capable of discriminating E. coli from other enteric bacteria, including its...
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Main Authors: | , , , |
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格式: | Artigo |
語言: | Inglês |
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American Society for Microbiology
2000
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在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC91908/ https://ncbi.nlm.nih.gov/pubmed/10653763 |
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