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In Situ Hybridization of Prochlorococcus and Synechococcus (Marine Cyanobacteria) spp. with rRNA-Targeted Peptide Nucleic Acid Probes

A simple method for whole-cell hybridization using fluorescently labeled rRNA-targeted peptide nucleic acid (PNA) probes was developed for use in marine cyanobacterial picoplankton. In contrast to established protocols, this method is capable of detecting rRNA in Prochlorococcus, the most abundant u...

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Bibliographic Details
Main Authors: Worden, Alexandra Z., Chisholm, Sallie W., Binder, Brian J.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC91819/
https://ncbi.nlm.nih.gov/pubmed/10618237
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