Engineering of a Stable Whole-Cell Biocatalyst Capable of (S)-Styrene Oxide Formation for Continuous Two-Liquid-Phase Applications

Recombinant strains of Pseudomonas putida KT2440 carrying genetic expression cassettes with xylene oxygenase- and styrene monooxygenase-encoding genes on their chromosomes could be induced in shaking-flask experiments to specific activities that rivaled those of multicopy-plasmid-based Escherichia c...

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Bibliographic Details
Main Authors: Panke, Sven, de Lorenzo, Víctor, Kaiser, Arnë, Witholt, Bernard, Wubbolts, Marcel G.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1999
Subjects:
Physiology and Biotechnology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC91770/
https://ncbi.nlm.nih.gov/pubmed/10584030
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