Dephosphorylation of Phytate by Using the Aspergillus niger Phytase with a High Affinity for Phytate

A phytase (EC 3.1.3.8) with a high affinity for phytic acid was found in Aspergillus niger SK-57 and purified to homogeneity in four steps by using ion-exchange chromatography (two types), gel filtration, and chromatofocusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Nagashima, Tadashi, Tange, Tatsuya, Anazawa, Hideharu
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91625/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10508107/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.10.4682-4684.1999
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