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A Sensitive Nested Reverse Transcriptase PCR Assay To Detect Viable Cells of the Fish Pathogen Renibacterium salmoninarum in Atlantic Salmon (Salmo salar L.)

A nested reverse transcriptase (RT) PCR assay detected mRNA of the salmonid pathogen Renibacterium salmoninarum in samples of RNA extracts of between 1 and 10 cells. Total RNA was extracted from cultured bacteria, Atlantic salmon (Salmo salar L.) kidney tissue and ovarian fluid seeded with the patho...

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Bibliografiske detaljer
Main Authors: Cook, Marcia, Lynch, William H.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 1999
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC91454/
https://ncbi.nlm.nih.gov/pubmed/10388701
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