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High-Level Production of Human Leptin by Fed-Batch Cultivation of Recombinant Escherichia coli and Its Purification

Human leptin is a 16-kDa (146-amino-acid) protein that is secreted from adipocytes and influences body weight homeostasis. In order to obtain high-level production of leptin, the human obese gene coding for leptin was expressed in Escherichia coli BL21(DE3) under the strong inducible T7 promoter. Th...

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書目詳細資料
發表在:Appl Environ Microbiol
Principais autores: Jeong, Ki Jun, Lee, Sang Yup
格式: Artigo
語言:Inglês
出版: American Society for Microbiology (ASM) 1999
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在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91452/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10388699/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.7.3027-3032.1999
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