Deletions in the Gibberellin Biosynthesis Gene Cluster of Gibberella fujikuroi by Restriction Enzyme-Mediated Integration and Conventional Transformation-Mediated Mutagenesis

We induced mutants of Gibberella fujikuroi deficient in gibberellin (GA) biosynthesis by transformation-mediated mutagenesis with the vector pAN7-1. We recovered 24 GA-defective mutants in one of nine transformation experiments performed without the addition of a restriction enzyme. Each mutant had...

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Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	Linnemannstöns, Pia, Voß, Thorsten, Hedden, Peter, Gaskin, Paul, Tudzynski, Bettina
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1999
Fag:	Genetics and Molecular Biology
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91378/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10347043/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.6.2558-2564.1999
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