A Double-Selective Tissue Culture System for Isolation of Wild-Type Poliovirus from Sewage Applied in a Long-Term Environmental Surveillance

We describe a simple, cost-efficient, double-selective method for isolation of wild-type poliovirus from sewage samples containing vaccine polioviruses and other enteroviruses, with a detection limit of 18 to 50 PFU per 1 to 2 liters of sewage. By this method we were able to process 1,700 sewage sam...

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Bibliographic Details
Main Authors: Manor, Y., Handsher, R., Halmut, T., Neuman, M., Abramovitz, B., Mates, A., Mendelson, E.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1999
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC91255/
https://ncbi.nlm.nih.gov/pubmed/10103285
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