A Double-Selective Tissue Culture System for Isolation of Wild-Type Poliovirus from Sewage Applied in a Long-Term Environmental Surveillance

We describe a simple, cost-efficient, double-selective method for isolation of wild-type poliovirus from sewage samples containing vaccine polioviruses and other enteroviruses, with a detection limit of 18 to 50 PFU per 1 to 2 liters of sewage. By this method we were able to process 1,700 sewage sam...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Manor, Y., Handsher, R., Halmut, T., Neuman, M., Abramovitz, B., Mates, A., Mendelson, E.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Methods
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91255/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10103285/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.4.1794-1797.1999
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