Quantitative Selective PCR of 16S Ribosomal DNA Correlates Well with Selective Agar Plating in Describing Population Dynamics of Indigenous Pseudomonas spp. in Soil Hot Spots

We used a quantitative PCR method targeting 16S ribosomal DNA using competitive PCR for specific detection of indigenous Pseudomonas DNA in soil hot spots. The amount of Pseudomonas DNA corresponded to the number of culturable Pseudomonas bacteria on Gould’s S1 agar. This represents the first use of...

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Hauptverfasser: Johnsen, Kaare, Enger, Øivind, Jacobsen, Carsten S., Thirup, Laila, Torsvik, Vigdis
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1999
Schlagworte:
General Microbial Ecology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC91253/
https://ncbi.nlm.nih.gov/pubmed/10103283
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