Biphasic Extracellular Proteolytic Enzyme Activity in Benthic Water and Sediment in the Northwestern Mediterranean Sea

In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog (l-leucine-7-amido-4-methylcoumarin) to determine the maximal ectoproteolytic activities (V(m)) and affinities (K(m)) of natural benthic microbial communities by the multiconcentration kinetic method. Thi...

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Detalles Bibliográficos
Main Authors: Tholosan, Olivier, Lamy, François, Garcin, Jean, Polychronaki, Thalia, Bianchi, Armand
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1999
Assuntos:
General Microbial Ecology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC91229/
https://ncbi.nlm.nih.gov/pubmed/10103259
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