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Biphasic Extracellular Proteolytic Enzyme Activity in Benthic Water and Sediment in the Northwestern Mediterranean Sea
In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog (l-leucine-7-amido-4-methylcoumarin) to determine the maximal ectoproteolytic activities (V(m)) and affinities (K(m)) of natural benthic microbial communities by the multiconcentration kinetic method. Thi...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology
1999
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC91229/ https://ncbi.nlm.nih.gov/pubmed/10103259 |
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