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Inhibition of Plant-Pathogenic Fungi by a Corn Trypsin Inhibitor Overexpressed in Escherichia coli

The cDNA of a 14-kDa trypsin inhibitor (TI) from corn was subcloned into an Escherichia coli overexpression vector. The overexpressed TI was purified based on its insolubility in urea and then refolded into the active form in vitro. This recombinant TI inhibited both conidium germination and hyphal...

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Autori principali: Chen, Zhi-Yuan, Brown, Robert L., Lax, Alan R., Cleveland, Thomas E., Russin, John S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 1999
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC91182/
https://ncbi.nlm.nih.gov/pubmed/10049901
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