High-Level Formation of Active Pseudomonas cepacia Lipase after Heterologous Expression of the Encoding Gene and Its Modified Chaperone in Escherichia coli and Rapid In Vitro Refolding

The lipase from Pseudomonas cepacia ATCC 21808 (recently reclassified as Burkholderia cepacia) is widely used by organic chemists for enantioselective synthesis and is manufactured from recombinant P. cepacia harboring on a plasmid the clustered genes for lipase and its chaperone. High levels of exp...

Полное описание

Библиографические подробности

Опубликовано в::	Appl Environ Microbiol
Главные авторы:	Quyen, Dinh Thi, Schmidt-Dannert, Claudia, Schmid, Rolf D.
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 1999
Предметы:	Physiology and Biotechnology
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC91096/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9925617/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.65.2.787-794.1999
Метки:	Добавить метку Нет меток, Требуется 1-ая метка записи!